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recombinant dna technology applications dna technology applications recombinant dna technology solutions
recombinant dna technology applications
recombinant dna technology solutions
Bioasset Technologies Pvt. Ltd.
Bioasset Technologies Pvt. Ltd.
Bioasset Technologies Pvt. Ltd.
Bioasset Technologies Pvt. Ltd.
Bioasset Technologies Pvt. Ltd.
Bioasset Technologies Pvt. Ltd.
Bioasset Technologies Pvt. Ltd.
Bioasset Technologies Pvt. Ltd.
Bioasset Technologies Pvt. Ltd.
recombinant dna technology solutions



recombinant dna technology solutions
dna technology applications








recombinant dna technology solutions
dna technology applications

Bioasset Technology Transfers



We represent established and reliable overseas companies for both classical fermentation product technologies and for rDNA therapeutic protein technologies. These companies are expert in their respective areas.

The rDNA technologies are designed for expressing recombinant native human proteins, having very high value as biopharmaceuticals. Having the knowledge of rDNA technology, we are able to suggest, guide, take you through the technology transfer process and further assist you in technology implementation if desired.

Microbiology And Biotechnology

Some of the salient features of the rDNA technologies that we offer are:

  • Wide range of products
  • High yielding r-DNA clones.
  • Cost effective purification process
  • Training at technology provider's facility abroad
  • More than 99% purity of final protein
  • Back-up and support for implementation.

Technology Development



Brief Description Of rDNA Technology Transfer Package

1. Manufacturing
2. Biosynthesis of the protein substance

  • General.
  • Construction of the plasmid expressing of the protein.
  • DNA sequence.
  • Expression of the protein in E.coli.

  • Fermentation process.
  • Inoculum.
  • Seed fermentor.
  • Production fermentor.
  • Fermentor Harvest.
  • Cell disruption.
  • Water Washes.


3. Purification of the protein from inclusion bodies
4. Detailed characterization of the protein

  • Dissolution of the protein inclusion bodies.
  • Dilution and refolding of the protein.
  • Clarification and concentration.
  • Enzymatic removal of the N-terminal methionine (if needed).
  • Purification of the protein by preparative RP-HPLC (if needed).
  • Purification of the protein by ion-exchange Chromatography (if needed).
  • Dialysis and Lyophilization.

  • Polyacrylamide gel electrophoresis.
  • UV spectroscopy.
  • N-terminal amino acid sequence determination.
  • Analytical reversed-phase HPLC.
  • Biological activity in-vitro.


5. Characterization of impurities

  • Endotoxin content by LAL.


Bioasset Technologies Pvt. Ltd.


The rDNA Technologies that we offer are:
Interferon Alpha 2a & 2b, Interferon Beta, Interleukins, G-CSF, GM-CSF, EGF, HGH, EPO, Insulin and many others.

Microbiology And Biotechnology
We also offer classical fermentation technologies for

1. Cyclosporin A,
2. Clavulanic acid,
3. Erythromycin,
4. Hyaluronic acid (HA),
5. Vancomycin,
6. Lovastatin,
7. Rapamycin,
8. Natamycin,
9. Itaconic Acid,
10. Shikimic Acid,
11. Vitamin B12,
12. Glutathione,
13. Carotin,
14. Xanthan .
and many more....
Bioasset Technologies Pvt. Ltd.


Bioasset Contract Manufacturing


Technology Transfer Bioassests
For overseas clients, India is a major player in pharma generics and also for off-patent biotech rDNA protein manufacturing.

Having excellent infrastructure which complies with the stringent GMP standards and with competent people to handle the job the only issue is the cost, which by all western standards is attractively low.

We being in the core area of Biotechnology and Pharmaceuticals are associated with reliable companies who carry out manufacturing for pharma and biotech industries.

We provide you the right partner for outsourcing the manufacturing. After due evaluation of the facility and staff, you may enter into contract with these companies for your products. If you require monitoring the process, time to time and also performing quality audits, we can extend the necessary support to you.

We provide contract manufacturing for non-sterile dosages, including powders, capsules, tablets, ointments and liquid filling.

We also offer contract manufacturing for sterile dosage forms.

As regards fermentation based products, our range covers from small scale fermentations of 10 L to 50KL.

We have excellent tie-ups for rDNA manufacturing including the DSP.

Bioasset Technologies Pvt. Ltd.




Bioasset Technologies Pvt. Ltd.
Our Services
Biopharmaceutical Services Project Feasibility Reports Clean Room Technology Microbiological Evaluation & Testing
Technology Transfers Contract Manufacturing Facility Design Laboratory Design & Furniture
Biopharmaceutical Technology Development Turnkey Projects
Bioasset Technologies Pvt. Ltd.
   
Product Range of Bioasset Equipments
Autoclaves/Steam Sterilizers Biosafety Cabinet Blood Bank Equipment Centrifuges Clean Air System
Cloud & Pour Point Deep Freezer Environmental Chamber Fermentors High Temperature Bath
Laboratory Oven Hot Air Sterilizer Incubators Laminar Air Flow Laboratory Refrigerator
Lyophilizer (Freeze Drier) Rotary Shakers Stability Test Chambers Ultra Cryostatic Circulator Ultrasonic Cleaner
Water Bath Boiler Laboratory Furniture Clean Room Panelling  
   
   

Bioasset Technologies Pvt. Ltd.
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Bioasset Technologies Pvt. Ltd.